Publication details
Identification of bacterial strains of species Lactococcus lactis in hard cheese using PCR
| Authors | |
|---|---|
| Year of publication | 2008 |
| Type | Article in Proceedings |
| Conference | 4th Meeting on Chemistry and Life, Chemické listy,102 (2008) No15 |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | cheese; Lactococcus lactis; P(HEMA-co-EDMA); PCR; |
| Description | PCR-ready DNA was isolated from different type of cheese using magnetic microspheres P(HEMA-co-EDMA) containing carboxyl groups in the presence of high concentrations of PEG 6000 and NaCl. The quality of extracted DNA was checked by PCR amplification. Species-specific PCR primers PALA4 and PALA14 (targeted on acm A gene encoding N-acetylmuramidase specific to Lactococcus lactis, 1131 bp long PCR products ) were used for the identification of Lactococcus lactis species with success. |
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