Publication details

Monitoring of in vitro DNA synthesis and PCR amplification using 7-deaza-purines as electroactive markers

Authors

FOJTOVÁ Miloslava HORÁKOVÁ Petra PIVOŇKOVÁ Hana ŠIMKOVÁ Eva VYCHODILOVÁ Zdenka HAVRAN Luděk FOJTA Miroslav

Year of publication 2009
Type Article in Proceedings
Conference Moderní elektrochemické metody
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords 7-deaza purines; electrochemical detection; carbon electrode; repetitive elements
Description 7 deaza purines (particularly 7 deaza guanine) incorporated into the DNA sequence during in vitro extension and/or PCR enable the simple electrochemical detection of the PCR products at the carbon eletrode. Based on the pilot experiments, the utilization of the method in a fast and simple analysis of G rich repetitive sequences in eukaryotic genomes is supposed.
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