Publication details
Monitoring of in vitro DNA synthesis and PCR amplification using 7-deaza-purines as electroactive markers
Authors | |
---|---|
Year of publication | 2009 |
Type | Article in Proceedings |
Conference | Moderní elektrochemické metody |
MU Faculty or unit | |
Citation | |
Field | Genetics and molecular biology |
Keywords | 7-deaza purines; electrochemical detection; carbon electrode; repetitive elements |
Description | 7 deaza purines (particularly 7 deaza guanine) incorporated into the DNA sequence during in vitro extension and/or PCR enable the simple electrochemical detection of the PCR products at the carbon eletrode. Based on the pilot experiments, the utilization of the method in a fast and simple analysis of G rich repetitive sequences in eukaryotic genomes is supposed. |
Related projects: |