Publication details
Ten new temperate bacteriophages of Citrobacter youngae.
| Authors | |
|---|---|
| Year of publication | 2004 |
| Type | Article in Periodical |
| Magazine / Source | Folia microbiologica |
| MU Faculty or unit | |
| Citation | |
| web | http://www.cssm.info/priloha/fm2004_671.pdf |
| Field | Microbiology, virology |
| Keywords | DNA HYBRIDIZATION; SP-NOV; CLASSIFICATION; BACTERIOCIN; STRAINS; PHAGES |
| Description | In a cross-test, we examined 55 strains of Citrobacter youngae against each other as potential producers of temperate bacteriophages and as potential sensitive indicators for them. Ten strains (18.2%) showed the production of phages. Seven different strain-specific spectra of activity (from 1 to 11 strains each) were found. Phage production by 6 strains was inducible with mitomycin C, in 4 strains it was not inducible. Using a polyclonal, specific anti-lambda serum, all 10 phages were found to be clearly distinct from E. coli lambda phage, the phage 31/47 showing the highest neutralization titre of all. Five phage-immune clones lysogenized with 5 of the phages kept their remaining phage sensitivity spectra, though extended by sensitivity to 1-3 phages.The phages belong to the morphotypes of Myoviridae (6 phages) and Siphoviridae (4 phages), with head diameters of 51-58 nm and tail length of 97-173 nm. Three strains produced corpuscular bacteriocins. |
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