Publication details
Identifikace laktobacilů izolovaných z potravin pomocí PCR a MALDI-TOF MS
| Title in English | Identification of lactobacilli isolated from food by PCR and MALDITOF MS |
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| Authors | |
| Year of publication | 2010 |
| Type | Article in Proceedings |
| Conference | XII. Konference mladých vědeckých pracovníků s mezinárodní účastí |
| MU Faculty or unit | |
| Citation | |
| Field | Microbiology, virology |
| Description | The aim of this study was to compare two methods for the species identification. 175 strains of lactobacilli isolated from milk and meat products (n = 139) were identified by genotypic and chemotaxonomic methods: the polymerase chain reaction (PCR) and the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Using the PCR method strains were differentiated into 10 species and into 16 species by MALDI-TOF MS. The total agreement of both methods has been observed in five species (L. curvatus, L. delbrueckii, L. johnsonii, L. plantarum a L. sakei). |
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