Publication details
RETENTION BEHAVIOUR OF OLIGONUCLEOTIDES ISOMERS STUDIED BY MEANS OF HYDROPHILIC INTERACTION CHROMATOGRAPHY
| Authors | |
|---|---|
| Year of publication | 2010 |
| Type | Article in Proceedings |
| Conference | X. pracovní setkání fyzikálních chemiků a elektrochemiků - sborník příspěvků |
| MU Faculty or unit | |
| Citation | |
| Field | Analytic chemistry |
| Keywords | oligonucleotides; chromatography |
| Description | Short oligonucleotides and its isomers are deeply studied for better understanding of nucleic acid properties and interactions. High performance liquid chromatography (HPLC) provides suitable tool for their characterization. Our interest in this study was focused on the separation of pentamers, consisted of adenine, guanine and thymine by means of hydrophilic liquid chromatography mode (HILIC). The influence of experimental parameters such as mobile phase pH and/or buffer concentration, percentage of organic solvent in mobile phase and flow rate was evaluated and the most acceptable conditions were selected for further analysis. Finally, obtained results were compared with commonly applied reverse phase liquid chromatography (RP HPLC). |
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