SINGLESTRAND CONFORMATION POLYMORPHISM OF OLIGONUCLEOTIDES IN THEIR ISOLATION BY MEANS OF MAGNETIC MICROPARTICLES

• Authors: TRNKOVÁ Libuše; FOJTÍKOVÁ Martina; BARTOŠKOVÁ Jana; HOLUBOVÁ Sylvie; KIZEK René
• Year of publication: 2010
• Type: Article in Proceedings
• Conference: X. pracovní setkání fyzikálních chemiků a elektrochemiků - sborník příspěvků
• MU Faculty or unit: Faculty of Science
• Field: Physical chemistry and theoretical chemistry
• Keywords: conformation polymorphism;oligonucleotides; magnetic particles
• Description: Studies of biomolecules depend strongly on efficiency of their isolation from biological materials. Using paramagnetic particles (MPs) represent new method for these purposes. This method, faster and less laborious than commonly used phenol chloroform extraction, is based on hybridization of target molecules on a surface of particles. For the isolation of nucleic acids MPs are modified by certain sequences of thymine or other nucleotides. Using MPs we studied the isolation of ODNs with different sequences of adenine and cytosine and ODNs with different chain lengths. It was found that singlestrand conformation polymorphism (SSCP) of ODNs is crucial phenomena for their isolation.

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