Využití paramagnetických částic pro izolaci oligonukleotidů s různou sekvencí a délkou řetězce

• Title in English: Use of paramagnetic particles for the isolation of oligonucleotides with different sequence and chain length
• Authors: FOJTÍKOVÁ Martina; BARTOŠKOVÁ Jana; HOLUBOVÁ Sylvie; KALETOVÁ Eva; TRNKOVÁ Libuše; KIZEK René
• Year of publication: 2010
• Type: Article in Proceedings
• Conference: XXX. moderní elektrochemické metody - sborník přednášek
• MU Faculty or unit: Faculty of Science
• Field: Biochemistry
• Keywords: Paramagnetic particles (MPs); Oligonucleotide; Adenine; Cytosine;Thymine; Adsorptive transfer stripping; Linear sweep voltammetry
• Description: Studies of biomolecules strongly depend on efficiency of their isolation from biological materials. Using paramagnetic particles (MPs) represent new method for these purposes. This method is based on hybridization of target molecules on a surface of particles. MPs can be modified with different molecules, for example sequences of thymine or other nucleotides for isolation of nucleic acids. This method is faster and less laborious then commonly used phenol chloroform extraction. Using MPs we studied the isolation of ODN with different sequence and chain length

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