ELEKTROCHEMICKÁ DETEKCE mRNA IZOLOVANÉ Z ROSTLINNÝCH PLETIV ZA VYUŽITÍ PARAMAGNETICKÝCH MIKROČÁSTIC

• Title in English: Electrochemical Detection of mRNA Isolated from Plant Tissues Using Paramagnetic Microparticles
• Authors: HÚSKA Dalibor; ADAM Vojtěch; TRNKOVÁ Libuše; KIZEK René
• Year of publication: 2010
• Type: Article in Periodical
• Magazine / Source: Chemické Listy
• MU Faculty or unit: Faculty of Science
• Field: Electrochemistry
• Keywords: ELIMINATION VOLTAMMETRY; DNA SENSORS; NUCLEIC-ACIDS; LINEAR SCAN; OLIGONUCLEOTIDES; HYBRIDIZATION; BIOSENSORS; RESOLUTION; REDUCTION; PROTEIN
• Description: The aim of this study was to utilize paramagnetic microparticles in isolation of mRNA using electrochemical detection. In this way mRNA from maize seedlings treated with a Cd(II) salt was isolated. The interaction of paramagnetic particles with synthetic oligo and polynucleotides of various chain lengths was investigated. The average detection limits were 0.020 to 0.360 nM and the quantification limits 0.080 to 1.200 nM.

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