Mutp53 non-B DNA structure binding to intronic sequences modulates gene expression in U251 cells

• Authors: TICHÝ V.; BRÁZDOVÁ Marie; QUANTE T.; TOGEL L.; WALTER K.; LOSCHER G.; NAVRATILOVA L.; LEXA Matej; DEPPERT W.; TOLSTONOG G.V.
• Year of publication: 2009
• Type: Conference abstract
• MU Faculty or unit: Faculty of Informatics
• Description: Missense point mutations in the TP53 gene are frequent genetic alterations in human tumor tissue and cell lines derived thereof. Mutant p53 (mutp53) proteins have lost sequence-specific DNA binding, but have retained the ability to interact in a structure-selective manner with non-B DNA and to act as regulators of transcription. We propose a model that attributes the oncogenic functions of mutp53 to its ability to interact with intronic and intergenic non-B DNA sequences and modulate gene transcription via re-organization of chromatin.

Related projects

• In vitro and in silico identification of non-canonical DNA structures in genomic sequences