Publication details

DNA barcoding of fungi: a case study using ITS sequences for identifying aquatic hyphomycete species

Authors

SEENA Sahadevan PASCOAL Cláudia MARVANOVÁ Ludmila CÁSSIO Fernando

Year of publication 2010
Type Article in Periodical
Magazine / Source Fungal Diversity
MU Faculty or unit

Faculty of Science

Citation
Field Microbiology, virology
Keywords Aquatic hyphomycetes - ITS - Barcode - Evolutionary divergence
Description Aquatic hyphomycetes are a polyphyletic group of fungi that play a crucial role in organic matter turnover in streams. They have been traditionally identified based on the morphology of conidia collected in stream water or obtained from leaves colonized in nature upon aeration in the laboratory. Currently, a great effort in fungal taxonomy is being made at introducing a set of criteria based on comparisons of selected nucleotide sequences instead of or in addition to phenotypic characters. We examined the suitability of ITS1-5.8S-ITS2 rRNA gene region or its subregions (ITS1 and ITS2) to identify aquatic hyphomycetes and we therefore propose ITS1-5.8S-ITS2 rRNA gene as barcode for identifying species of aquatic hyphomycetes.
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