Publication details
Průkaz těžkých kovů v ledvinách fétů po perorálním podávání olova, rtuti a kadmia březím myším samicím
| Title in English | Distribution of heavy metals in the kidneys of foetuses after peroral administration of lead, mercury and cadmium to pregnant female mice |
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| Authors | |
| Year of publication | 2010 |
| Type | Article in Proceedings |
| Conference | Plzeňský lékařský sborník |
| MU Faculty or unit | |
| Citation | |
| Field | Morphological specializations and cytology |
| Keywords | Heavy metals; mouse; foetus; kidneys |
| Description | Distribution of heavy metals and of subsequently developed morphological changes in the kidneys of mouse foetuses after peroral administration of high doses of lead, mercury, and cadmium (0.03 mg of metal per mouse and day) was studied. Heavy metals were administered to pregnant female mice on days 9 to 20 of pregnancy. The animals were euthanised by cervical dislocation. Samples of foetal kidneys were subsequently removed and processed standardly for purposes of electron microscopy. Histochemical reaction based on metal conversion into appropriate sulphide that conjugates with silver was used for detection of heavy metals. Deposits of heavy metals were found at the proximal and distal tubules of kidneys. At the electron microscopy level, accumulation of heavy metals was connected as a rule with the occurrence of a certain damage to some organelles. Deposits of the reaction product were located above all in epithelial cells. Heavy metals were bound to the heterochromatin of cell nuclei, as well as to some cytoplasmic organelles, such as rough endoplasmic reticulum, mitochondria and ribosomes. The presence of heavy metals was associated with obliteration of cisternae of the rough endoplasmic reticulum and separation of ribosomes. Vacuolation of cell cytoplasm was also a frequent phenomenon. Observed changes era possible label as signs tubular necrosis. An interesting finding was the "contrasting" of structures containing nucleic acids. |
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