Publication details
Formation and properties of the fibrillar network formed in yeast protoplasts as the first step of biosynthesis of the cell wall.
| Authors | |
|---|---|
| Year of publication | 1967 |
| Type | Article in Proceedings |
| Conference | In: “SYMPOSIUM ON YEAST PROTOPLASTS“. Jena 21.-24.9.1965. |
| MU Faculty or unit | |
| Citation | |
| Web | http://apps.isiknowledge.com/summary.do?product=WOS&search_mode=CitedReferenceSearch&qid=1&SID=T1m2N5@EHN@J@mbp4PC&TitleFormat=full&page=1 |
| Field | Morphological specializations and cytology |
| Description | Yeast protoplasts cultivated in liquid media are able to form a system of fibrils only. Fibril network can be identified after lyses of protoplasts by distilled water. Isolated network consists of micro fibrils of an average thickness about 20 nm that are arranged in flat bundles. The presence of snail enzymes in culture medium blocked the formation fibril nets. Application of PAS reaction according to Mundkur showed that isolated fibrillar nets remained PAS negative. As far as solubility of fibril nets in acids and alkaline, fibril nets were soluble in 1 N NaOH at 70 degrees C, but they were resistant towards acids. Resistance of fibrillar nets to snail enzymes showed that the fibrils formed on the surface of protoplasts are not quite identical – as far as their physical properties – to those that are supposed to form the groundwork of the cell wall. It is assumed that the fibrils are composed of acid insoluble, but alkali soluble glucan. |