Publication details
Characterization of a fission yeast mutant which displays defects in cell wall integrity and cytokinesis
| Authors | |
|---|---|
| Year of publication | 2001 |
| Type | Article in Periodical |
| Magazine / Source | Genes Genet. Syst. |
| MU Faculty or unit | |
| Citation | |
| Web | http://apps.isiknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&%20qid=1&SID=P2bki1ma@7PHigdNPFC&page=1&doc=8 |
| Field | Genetics and molecular biology |
| Keywords | BINDING PROTEIN RHO1; SCHIZOSACCHAROMYCES POMBE; SACCHAROMYCES CEREVISIAE; GENE ENCODES; REGENERATING PROTOPLASTS; 1 3-BETA GLUCAN SYNTHASE; ACTIN CYTOSKELETON; SEPTUM FORMATION; SHAPE CONTROL; S. POMBE |
| Description | The fission yeast cps6-153 mutant was originally isolated based on its hypersensitivity to the spindle poison isopropyl N-3-chlorophenyl carbamate (CIPC). The mutant also shows defects in both cell wall integrity and cytokinesis, resulting in the acccumulation of unseparated cells with weakened cell walls. The arrested cells display a disoriented alignment of cytoplasmic microtubules. When the mutant cells are cultivated at high temperature (35 degrees C), both cell walls and septa become very thick. Electron microscopy revealed the disorganized structure of the thickened cell walls and septa, in which fibrillar components were not completely masked with an amorphous matrix. rad25+ was cloned from a genomic library by complementation of the mutant phenotypes, suggesting the involvement of Rad25p, one of two 14-3-3 proteins in S. pombe, in the pathway of cell wall integrity and cytokinesis. |
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