Publication details
Electron microscopic study of purified polysaccharide components glucans and mannan of the cell walls in the yeast Saccharomyces cerevisiae
| Authors | |
|---|---|
| Year of publication | 1985 |
| Type | Article in Periodical |
| Magazine / Source | J. Basic Microbiol. |
| MU Faculty or unit | |
| Citation | |
| Web | http://apps.isiknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=1&SID=P1k82D9jd8LP@JPdmLi&page=3&doc=24 |
| Field | Microbiology, virology |
| Keywords | yeast cell wall; purified wall polysaccharides; electron microscopy |
| Description | In this study electron-microscopic characteristics of platinum shadowed or negatively stained preparations of purified beta-1,3-d-glucan, beta-1,6-d-glucan, and mannan were examined. These polysaccharides were isolated from cell walls of the yeast Saccharomyces cerevisiae. While purified samples of beta-1, 6-d-glucan and mannan proved to be amorphous in structure and homogenous in appearance, the purified beta-1, 3-d-glucan, isolated and presented to us as alkali-insoluble yeast glucan A2, was not homogenous. It consisted of (i) fibrillar component, (ii) amorphous matrix, and (iii) chitin bud scars. The ultrastructure of beta-1, 3-d-glucan present in the glucan A2 sample did not change after treatment with 0.5 m acetic acid at 75 degrees C for 2 hours. After treatment with 1 m NaOH for 3 days at 4 degrees C scar material was removed by centrifugation and after a subsequent acidification of supernatant with acetic acid both the microfibrillar and the amorphous components were still present. It was concluded that beta-1, 3-d-glucan component consists of molecules probably differing in their physico-chemical properties such as D. P., the degree of branching, conformation, and that cannot be separated by the methods currently used for their isolation. |