Publication details
Regeneration of the cell wall of protoplasts of the fission yeast Schizosaccharomyces versatilis in liquid media and their reversion to cells.
| Authors | |
|---|---|
| Year of publication | 1983 |
| Type | Article in Periodical |
| Magazine / Source | Folia microbiol. |
| MU Faculty or unit | |
| Citation | |
| Web | http://apps.isiknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=1&SID=U1JM4IOhk@KkBjc4PDL&page=3&doc=29 |
| Field | Microbiology, virology |
| Keywords | protoplasts; Schizosaccharomyces versatilis; cell wall regeneration;light and electron microscopy |
| Description | Regeneration of the cell wall and reversion of protoplasts with a completely regenerated cell wall to cells were studied by light and electron microscopy in protoplasts of the fission yeast Schizosaccharomyces versatilis. On their surface the protoplasts regenerated a complete new wall even in liquid media. The wall regeneration began with the formation of a thin irregular net of flat bundles of long microfibrils and the net was gradually filled with aggregates of short straight microfibrils and small piles of amorphous material. Osmotically resistant organisms with regenerated walls were detected after a 4–6 h cultivation. Depending on the nutrient medium used 10–80 % of protoplasts with the regenerated wall were obtained that reverted subsequently to cells. The high percentage of the wall regeneration and reversion to cells was reached by combining cultivation in a poor medium with that in a rich medium Reversion to cells could only occur after the protoplasts had regenerated rigid cell walls. These walled protoplasts underwent septation, and, by polar growth, produced cylindrical cells, further dividing by fission. |