Publication details

Staining the nuclei in cells and protoplasts of living yeasts, moulds and green algae with the antibiotic lomofungin.

Authors

KOPECKÁ Marie GABRIEL Miroslav

Year of publication 1978
Type Article in Periodical
Magazine / Source Arch. Microbiol.
MU Faculty or unit

Faculty of Medicine

Citation
Web http://apps.isiknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=1&SID=U1JM4IOhk@KkBjc4PDL&page=4&doc=31
Field Morphological specializations and cytology
Keywords Cell nucleus - RNA polymerases; Lomofungin; Nuclear staining; Yeasts; Moulds; Green algae; Cytology
Description Lomofungin, an antibiotic active against yeasts, mycelial fungi and bacteria imparts a red colour to living yeast, fungal and algal nuclei when it is present in the culture medium at concentration of 20–100 micrograms/ml. Staining is equally distinct at all stages of the cell cycle, including mitosis and meiosis. The coloured nuclei are plainly visible in the light microscope and the presence in them of lomofungin has been confirmed by electron microscopy.

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