Publication details

A method of isolating anucleated yeast protoplasts unable to synthesize the glucan fibrillar component of the wall

Authors

KOPECKÁ Marie GABRIEL Miroslav NEČAS Oldřich

Year of publication 1974
Type Article in Periodical
Magazine / Source J. Gen. Microbiol.
MU Faculty or unit

Faculty of Medicine

Citation
Web http://apps.isiknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=1&SID=W1okoo1b85iLefnnN9I&page=5&doc=42
Field Microbiology, virology
Keywords anucleated yeast protoplasts; synthesis of glucan fibrillar network
Description A mixture of nucleated and anucleated protoplasts was produced from log-phase Saccharomyces cerevisiae by the use of snail enzymes. The mixture was separated by centrifugation, and anucleated protoplasts were studied by means of light and electron microscopy. Anucleated protoplasts did not synthesize glucan fibrils even though they seemed to contain all other basic structures in their cytoplasm, and the structure of the plasma membrane was unchanged. This was in sharp contrast to ordinary nucleated protoplasts which synthesized glucan fibrils even after inhibition of protein synthesis by cycloheximide. The reason for this behaviour of anucleated protoplasts is not clear. Such anucleated yeast protoplasts represent the first example of uniform anucleated fungi produced by a reproducible method.

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