Publication details
Nature of the fibrillar nets of Saccharomyces protoplasts in liquid media.
| Authors | |
|---|---|
| Year of publication | 1973 |
| Type | Chapter of a book |
| MU Faculty or unit | |
| Citation | |
| Description | The protoplast nets of S. cerevisiae have been found to contain, in contrast to native cell walls, free chains of beta-1,3-linked D-glucose residues aggregated to form crystalline structures. These structures form part of the long micro fibrils constituting the nets. The crystalline packing of the chains is slightly different from that of hydroglucan and corresponds to that of paramylum. The nets contain chitin which is formed de novo by the protoplasts and, in contrast to the chitin in the native walls, is crystalline. The chitin to glucan ratio of the nets is normally higher than in native walls. The net structure is disturbed by dilute alkali, which dissolves about 40 %, the residual part consisting of more or less microfibrillar clusters and chitin. The X-ray diagram of the clusters is indicative of the presence in them of beta-1,3-glucan chains, but their true nature has not been yet established. Then constitutional and structural differences between the nets and the glucan-chitin moiety of normal walls have tentatively been explained by differential removal of 1,6 and 1,3 linking enzyme and removal of a chitin completing factor from their natural locus of action by the liquid medium. |