Substrate Specificity of Haloalkane Dehalogenases

• Authors: KOUDELÁKOVÁ Táňa; CHOVANCOVÁ Eva; BREZOVSKÝ Jan; MONINCOVÁ Marta; FOŘTOVÁ Andrea; JARKOVSKÝ Jiří; DAMBORSKÝ Jiří
• Year of publication: 2011
• Type: Article in Periodical
• Magazine / Source: Biochemical Journal
• MU Faculty or unit: Faculty of Science
• Doi: http://dx.doi.org/10.1042/BJ20101405
• Field: Biochemistry
• Keywords: substrate specificity; haloalkane dehalogenases; halogenated compounds
• Description: An enzyme's substrate specificity is one of its most important characteristics. The quantitative comparison of broad-specificity enzymes requires the selection of a homogenous set of substrates for experimental testing, determination of substrate specificity data, and analysis using multivariate statistics. We describe a systematic analysis of the substrate specificities of nine wild-type and four engineered haloalkane dehalogenases. The enzymes were characterized experimentally using a set of 30 substrates selected using statistical experimental design from a set of nearly 200 halogenated compounds.

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