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Informace o publikaci
Substrate Specificity of Haloalkane Dehalogenases
Název česky | Substrátová specifita haloalkan dehalogenáz |
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Autoři | |
Rok publikování | 2011 |
Druh | Článek v odborném periodiku |
Časopis / Zdroj | Biochemical Journal |
Fakulta / Pracoviště MU | |
Citace | |
Doi | http://dx.doi.org/10.1042/BJ20101405 |
Obor | Biochemie |
Klíčová slova | substrate specificity; haloalkane dehalogenases; halogenated compounds |
Popis | An enzyme's substrate specificity is one of its most important characteristics. The quantitative comparison of broad-specificity enzymes requires the selection of a homogenous set of substrates for experimental testing, determination of substrate specificity data, and analysis using multivariate statistics. We describe a systematic analysis of the substrate specificities of nine wild-type and four engineered haloalkane dehalogenases. The enzymes were characterized experimentally using a set of 30 substrates selected using statistical experimental design from a set of nearly 200 halogenated compounds. |
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