Publication details
Efficiency of PCR-based methods in discriminating Bifidobacterium longum ssp. longum and Bifidobacterium longum ssp. infantis strains of human origin
| Authors | |
|---|---|
| Year of publication | 2011 |
| Type | Article in Periodical |
| Magazine / Source | Journal of Microbiological Methods |
| MU Faculty or unit | |
| Citation | |
| Doi | http://dx.doi.org/10.1016/j.mimet.2011.06.014 |
| Field | Genetics and molecular biology |
| Keywords | Bifidobacterium longum; PCR; ARDR; RAPD; rep-PCR |
| Description | Four molecular-biological methods (PCRs, random amplified polymorphic DNA (RAPD) method using 5 primers, repetitive sequence-based (rep)-PCR with BOXA1R and (GTG)5 primers and amplified ribosomal DNA restriction analysis (ARDRA)) and biochemical analysis were compared for the classification of B. longum strains isolated from the faeces of breast-fed healthy infants (25), healthy adults (3) and 2 collection strains. These methods together with arabionose/ melezitose fermentation analysis clearly differentiated only the collection strains into B. longum ssp. longum and B. longum ssp. infantis at the subspecies level. On the other hand, ARDRA analysis differentiated the strains into the B. longum/infantis subspecies using the cleavage analysis of genus-specific amplicon with just one enzyme, Sau3AI. Moreover, we propose a classification of the collection strains into the subspecies level: B. longum CCM 3764 as B. longum ssp. longum CCM 3764, B. longum ATCC 17930 as B. longum ssp. infantis ATCC 17930. |
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