Microfluidic robotic device coupled with electrochemical sensor field for handling of paramagnetic micro-particles as a tool for determination of plant mRNA.

• Authors: HUSKA Dalibor; ADAM Vojtěch; BABULA Petr; TRNKOVÁ Libuše; HUBÁLEK Jaromír; ZEHNÁLEK Josef; HAVEL Ladislav; KIZEK René
• Year of publication: 2011
• Type: Article in Periodical
• Magazine / Source: Microchim. Acta
• MU Faculty or unit: Faculty of Science
• web: https://link.springer.com/article/10.1007/s00604-011-0545-z
• Doi: https://doi.org/10.1007/s00604-011-0545-z
• Field: Physical chemistry and theoretical chemistry
• Keywords: Electrochemical detection; Microfluidics; Environmental stress; Cadmium ions; Paramagnetic particles
• Description: The expression of genes responsible for the biosynthesis of stress proteins corresponds to the exposition of an organism to abiotic and/or biotic stress. We utilize two types of paramagnetic particles for isolation of total mRNA from early somatic embryos of Norway Spruce (Picea abies /L./ Karst.) and maize plants (Zea mays L.) treated with cadmium(II) ions. The paramagnetic particles were evaluated for analysis of real samples, and polyadenine was used as a model mRNA. Various approaches (from non-automatic to fully automatic) were tested in terms of handling the particles.

Related projects

• Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)