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Microfluidic robotic device coupled with electrochemical sensor field for handling of paramagnetic micro-particles as a tool for determination of plant mRNA.
Autoři | |
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Rok publikování | 2011 |
Druh | Článek v odborném periodiku |
Časopis / Zdroj | Microchim. Acta |
Fakulta / Pracoviště MU | |
Citace | |
www | https://link.springer.com/article/10.1007/s00604-011-0545-z |
Doi | http://dx.doi.org/10.1007/s00604-011-0545-z |
Obor | Fyzikální chemie a teoretická chemie |
Klíčová slova | Electrochemical detection; Microfluidics; Environmental stress; Cadmium ions; Paramagnetic particles |
Popis | The expression of genes responsible for the biosynthesis of stress proteins corresponds to the exposition of an organism to abiotic and/or biotic stress. We utilize two types of paramagnetic particles for isolation of total mRNA from early somatic embryos of Norway Spruce (Picea abies /L./ Karst.) and maize plants (Zea mays L.) treated with cadmium(II) ions. The paramagnetic particles were evaluated for analysis of real samples, and polyadenine was used as a model mRNA. Various approaches (from non-automatic to fully automatic) were tested in terms of handling the particles. |
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