Publication details
Electrochemical detection of 8 - hydroxy - 2´-dexyguanosine isolated from a real sample by use of antibody - modified paramagnetic particles
| Authors | |
|---|---|
| Year of publication | 2011 |
| Type | Article in Proceedings |
| Conference | 11th International Nutrition and Diagnostics conference INDC 2011 |
| MU Faculty or unit | |
| Citation | |
| Field | Physical chemistry and theoretical chemistry |
| Keywords | guanosine;paramagnetic particles;elisa;differential pulse voltammetry |
| Description | Guanosine derivatives are important for diagnostic of oxidative DNA damamge. 8 - hydroxy - 2-deoxyguanosine (8-OHdG) is one of most abundant products of DNA oxidation. Its determination in biological fluids, e. g. urine is possible. Nevertheless, owing to its low concentration and matrix interferences (uric acid) use of HPLC without previous extraction is difficult. In this study we tested the isolation of 8-OHdG from a biological matrix by using the paramagnetic particles with antibody - modified surface. We used the sandwich ELISA principles for determination of molecules of 8-OHdG. The primary antibody was bound to a paramgnetic particle, then 8-OHdG was attached during the reaction in the sample. Then the secondary antibody with alkaline phosphatase was attached. Concentration of the analyte was determined from concentration of napthol, which is a product of alkaline phosphatase where napthyl phosphate acts product. Every step during whole process was conducted with a magnetic holder which held the magnetic particles with bound substrate in place in the test tube... |