Publication details
RealTime PCR detekce baktérií způsobujících nespecifické uretritidy
| Title in English | RealTime PCR detection of bacteria causing non-specific urethritis |
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| Authors | |
| Year of publication | 2012 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | The most common bacterial cause of non-specific uretritis is Chamydia trachomatis but also Ureplasma urealyticum / parvum and Mycoplasma genitalium / hominis. Ureaplasma and mycoplasma are frequently part of the normal microbial flora, however for the diseased by non-specific urethritis, ureplasmata creates up to half causes and the mycoplasma has direct participation on the development of nonspecific urethritis chlamidia in 18 – 45 %. The basic symptoms include a frequent urge to urination, vaginal discharge or discharges of the urethra, pain and dysuria. The cycle of infection is often asymptomatic. The treatment is based on peroral consumption of antibiotics. Untreated infections can result in serious reproductive disorders at both genders. The potentional diagnostic methods are serological or cultivating examinations. The most accurate method of determining the DNA diagnosis, out of which has recently been used primarily RealTime PCR. The RealTime detection can be performed from the clinical material such as urine, vaginal and cervical smear, sperm or smear from uretra. Our study has analyzed a total of 96 urine samples from sexually active college students. The isolation of total DNA from urine was performed according to the modified protocol using commercially available isolation kit UltraClean BloodSpin DNA Isolation Kit (MoBio, USA). For RealTime PCR detection were used diagnostics kits EliGene Mycoplasma hom/gen LC, EliGene Ureaplasma LC a EliGene Chlamydia trachomatis LC (ELISABETH PHARMACON, ČR). The set of analyzed samples were able to demonstrated the presence of the bacterial originator of Chlamidia trachomatis in just one case. The Ureaplasma urealyticum/parvum has been proved in nine analyzed urine samples. DNA Mycoplasma genitalium /hominis has not been discovered in the tested samples. Based on the comparison of data from the questionnaire can also be confirmed an asymptomatic course of infection in some probands positive for Ureaplasma urealyticum/parvum. |