![Jak na přijímačky](https://cdn.muni.cz/media/3633702/image_1.jpg?mode=crop¢er=0.5,0.5&rnd=133572412150000000&heightratio=0.5&width=278)
Informace o publikaci
Site-specific Analysis of Protein Hydration Based on Unnatural Amino Acid Fluorescence.
Autoři | |
---|---|
Rok publikování | 2015 |
Druh | Článek v odborném periodiku |
Časopis / Zdroj | Journal of the American Chemical Society |
Fakulta / Pracoviště MU | |
Citace | |
www | http://loschmidt.chemi.muni.cz/peg/wp-content/uploads/2015/04/jacs15.pdf |
Doi | http://dx.doi.org/10.1021/jacs.5b01681 |
Obor | Biochemie |
Klíčová slova | dehalogenases;fluorescence spectroscopy;molecular dynamics simulations |
Popis | Hydration of proteins profoundly affects their functions. We describe a simple and general method for site-specific analysis of protein hydration based on the in vivo incorporation of fluorescent unnatural amino acids and their analysis by steady-state fluorescence spectroscopy. Using this method, we investigate the hydration of functionally important regions of dehalogenases. The experimental results are compared to findings from molecular dynamics simulations. |
Související projekty: |