Informace o publikaci

Thermal and pH stabilities of i‐DNA: confronting in vitro experiments with models and in‐cell NMR data

Autoři

CHENG Mingpan QIU Dehui TAMON Liezel IŠTVÁNKOVÁ Eva VÍŠKOVÁ Pavlína AMRANE Samir GUÉDIN Aurore CHEN Jielin LACROIX Laurent JU Huangxian TRANTÍREK Lukáš SAHAKYAN Aleksandr B. ZHOU Jun MERGNY Jean-Louis

Rok publikování 2021
Druh Článek v odborném periodiku
Časopis / Zdroj ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www https://onlinelibrary.wiley.com/doi/10.1002/anie.202016801
Doi http://dx.doi.org/10.1002/anie.202016801
Klíčová slova DNA; i-motif; thermal stability; pH transition; intracellular stability
Popis Recent studies indicate that i-DNA, a four-stranded cytosine-rich DNA also known as the i-motif, is actually formed in vivo ; however, a systematic study on sequence effects on stability has been missing. Herein, an unprecedented number of different sequences (271) bearing four runs of 3-6 cytosines with different spacer lengths has been tested. While i-DNA stability is nearly independent on total spacer length, the central spacer plays a special role on stability. Stability also depends on the length of the C-tracts at both acidic and neutral pHs. This study provides a global picture on i-DNA stability thanks to the large size of the introduced data set; it reveals unexpected features and allows to conclude that determinants of i-DNA stability do not mirror those of G-quadruplexes. Our results illustrate the structural roles of loops and C-tracts on i-DNA stability, confirm its formation in cells, and allow establishing rules to predict its stability.
Související projekty:

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.

Další info