Informace o publikaci

In-gel digestion for mass spectrometric characterization of proteins and proteomes

Autoři

HAVLIŠ Jan SHEVCHENKO Andrej MANN Mathias TOMAS Henrik OLSEN Jesper V.

Rok publikování 2008
Druh Článek v odborném periodiku
Časopis / Zdroj Nature Protocols
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Obor Genetika a molekulární biologie
Klíčová slova ASSISTED-LASER-DESORPTION/IONIZATION; POLYACRYLAMIDE-GELS; NANOELECTROSPRAY; MODEL; SILAC; MS
Popis In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 10-year-old recipe by Shevchenko et al. has been optimized to increase the speed and sensitivity of analysis. The protocol is for the in-gel digestion of both silver and Coomassie-stained protein spots or bands and can be followed by MALDI-MS or LC-MS/MS analysis to identify proteins at sensitivities better than a few femtomoles of protein starting material.

Používáte starou verzi internetového prohlížeče. Doporučujeme aktualizovat Váš prohlížeč na nejnovější verzi.

Další info