Informace o publikaci

Complementation of 3D structure of delta subunit of RNA polymerase from Bacillus subtilis with description of internal motions in terms of reduced spectral density mapping

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KADEŘÁVEK Pavel DIEHL Carl PAPOUŠKOVÁ Veronika ŠANDEROVÁ Hana PADRTA Petr ŽÍDEK Lukáš KRÁSNÝ Libor SKLENÁŘ Vladimír AKKE Mikael

Rok publikování 2011
Druh Článek v odborném periodiku
Časopis / Zdroj MATERIALS STRUCTURE
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www http://www.xray.cz/ms/bul2011-1.htm
Obor Biochemie
Klíčová slova NMR; delta subunit; RNA polymerase; spectral density function
Popis RNA polymerases of Gram positive and Gram negative bacteria differ. The subunit composition in Gram positive bacteria includes two additional subunits. One of them is denoted delta-subunit. The structure of delta-subunit from Bacillus subtilis has been solved recently using methods of solution NMR [1] (PDB ID = 2KRC). Here, we extend the information about the structure by the basic characterization of its dynamics studied at two temperatures. The standard relaxation experiments (R1, R2, ssNOE) were performed and data were analyzed using reduced spectral density mapping as the most straightforward approach. The analysis reveals flexible residues in the central part of the sequence. It confirms expected independent stochastic movements in the C-terminal part of the molecule. Results also yield an evidence of a slow conformational exchange of several residues in the well-structured region of the protein.
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