Electrochemical DNA Sensors Based on Adsorptive and Elimination Techniques

• Autoři: TRNKOVÁ Libuše; JELEN František; ADAM Vojtěch; KIZEK René
• Rok publikování: 2011
• Druh: Konferenční abstrakty
• Fakulta / Pracoviště MU: Přírodovědecká fakulta
• Popis: Electrochemistry-based sensors offer good sensitivity, separation ability, low cost for the detection of ODNs and selected DNA sequences or of muteted genes associated with diseases. Numerous developed approaches, such as the platforms or direct or indirect electrochemistry DNA, specific redox indicator, or mediated charge transport, can be improved by the adsorptive stripping technique in connection with elimination voltammetry with linear scan (AdS EVLS). Here we verify our idea on the electrode systems of homo-and hetero-oligodeoxynuleotides (ODNs) including hairpins. The EVLS was applied to the resolution of reduction signals of adenine (A) and cytosine (C) in short synthetic ODNs. The Ads EVLS results showed that the elimination functions chosen are also useful for the study not only of cathodic but also of anodic processes, especially for the anodic processes of guanine. Due to AdS EVLS or AdTS EVLS we are able to evaluate the ODN structure changes on electrode surfaces.

Související projekty

• Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální , regionální a lokální úrovni
• Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu
• Vývoj metodologie a instrumentace analytické chemie pro studium složení a vlastností přírodních a syntetických materiálů