Project information
New possibilities of diagnostics of leukemia using DNA-microarrays
- Project Identification
- 1A8241
- Project Period
- 7/2004 - 12/2006
- Investor / Pogramme / Project type
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Ministry of Health of the CR
- Health of People (National Research Programme)
- MU Faculty or unit
- Faculty of Informatics
- Keywords
- DNA microarrays;histone modifications;inhibitors of deacetylases;chromatin structure;leukemia;histon
- Cooperating Organization
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Institute of Biophysics
- Responsible person doc. RNDr. Stanislav Kozubek, DrSc.
- Responsible person prof. MUDr. Jiří Mayer, CSc.
Using DNA microarrays, gene expression will be studied in leukemia, in control cell populations and in cell cultures. Changes will be correlated
to epigenetic characteristics of chromatin at the level of its modifications (methylation, acetylation). Structural characteristics of chromatin at sites with altered expression will be studied using fluorescence in situ hybridization (FISH), spectral microscopy and immunoFISH. Drugs with narrow spectrum of genes with altered expression wil be studied. Using ad vanced techniques of gene or protein visualization and top level confocal microscopy, the mechanisms of the induction and progression of leukemia will be investigated with the goal to suggest appropriate markers for diagnostics. We hope that our study of gene expression in parallel to epigenetics will allow us to propose target molecules of effective therapy.
Publications
Total number of publications: 19
2007
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New insights into gene positional clustering and its properties supported by large-scale analysis of various differentiation pathways
Genomics, year: 2007, volume: 89, edition: 1
2006
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Comparative transcriptome maps: a new approach to the diagnosis of colorectal carcinoma patients using cDNA microarrays
Clinical Genetics, year: 2006, volume: 69, edition: 3
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Evaluation of CD34+ - and Lin- -selected cells from peripheral blood stem cell grafts of patients with lymphoma during differentiation in culture ex vivo using a cDNA microarray technique
Experimental hematology, year: 2006, volume: 34, edition: 7
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Localization of genetic elements of intact and derivative chromosome 11 and 22 territories in nuclei of Ewing sarcoma cells
Journal of Structural Biology, year: 2006, volume: 155, edition: 3
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Transcription density map as a new tool for evaluation of various expression profiles: Ex vivo differentiation of CD34+ cells
Year: 2006, type: Conference abstract
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Vybrané aplikace technologie cDNA microarrays v onkologickém výzkumu
Klinická onkologie, year: 2006, volume: 19, edition: 2
2005
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Analýza obrazů získaných snímáním microarrays
Year: 2005, type: Conference abstract
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Aplikace FDR a FWER testovacích procedur na data ze studie kolorektálního karcinomu
Year: 2005, type: Conference abstract
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Combination of cDNA microarray technology and fluorescence leads to better understanding of relationships between gene expression and epigenetic regulation
Year: 2005, type: Conference abstract
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Důkaz klastrování genů na chromosomových doménách studiem diferenciací buněčných nádorových linií
Year: 2005, type: Conference abstract