Project information
New possibilities of diagnostics of leukemia using DNA-microarrays
- Project Identification
- 1A8241
- Project Period
- 7/2004 - 12/2006
- Investor / Pogramme / Project type
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Ministry of Health of the CR
- Health of People (National Research Programme)
- MU Faculty or unit
- Faculty of Informatics
- Keywords
- DNA microarrays;histone modifications;inhibitors of deacetylases;chromatin structure;leukemia;histon
- Cooperating Organization
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Institute of Biophysics
- Responsible person doc. RNDr. Stanislav Kozubek, DrSc.
- Responsible person prof. MUDr. Jiří Mayer, CSc.
Using DNA microarrays, gene expression will be studied in leukemia, in control cell populations and in cell cultures. Changes will be correlated
to epigenetic characteristics of chromatin at the level of its modifications (methylation, acetylation). Structural characteristics of chromatin at sites with altered expression will be studied using fluorescence in situ hybridization (FISH), spectral microscopy and immunoFISH. Drugs with narrow spectrum of genes with altered expression wil be studied. Using ad vanced techniques of gene or protein visualization and top level confocal microscopy, the mechanisms of the induction and progression of leukemia will be investigated with the goal to suggest appropriate markers for diagnostics. We hope that our study of gene expression in parallel to epigenetics will allow us to propose target molecules of effective therapy.
Publications
Total number of publications: 19
2005
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New practical tools for microarray data analysis
Year: 2005, type: Conference abstract
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Normalizační metody ve zpracování dat z DNA Microarrays
Year: 2005, type: Conference abstract
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Nuclear levels and patterns of histone H3 modification and HP1 proteins after inhibition of histone deacetylases
Journal of Cell Science, year: 2005, volume: 118, edition: 21
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Sledování změn genové exprese u pacientů s kolorektálním karcinomem pomocí cDNA microarrays technologie a jejich diagnostika pomocí transkripčních map
Year: 2005, type: Conference abstract
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Transcriptional profiling of human CD34+ cells during in vitro differentiation
Year: 2005, type: Conference abstract
2004
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Clustering of genes with similar regulation patterns during monocyte and granulocyte differentiation of HL-60 cells
Biophysics of the Genome, year: 2004
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Cytological study of DNA methylation and histone methylation patterns in human cell lines
Biophysics of the Genome, year: 2004
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Dynamics of HP1 transgene loci movement and silencing in living cells
Biophysics of the Genome, year: 2004
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From Jenalumar to Ultrafast Microscopy of Living Cells
Biophysics of the Genome, year: 2004