Project information
Proteins in metabolism and interaction of organisms with the environment
- Project Identification
- MSM0021622413
- Project Period
- 1/2005 - 12/2011
- Investor / Pogramme / Project type
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Ministry of Education, Youth and Sports of the CR
- Research Intents
- MU Faculty or unit
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Faculty of Science
- prof. RNDr. Jaroslav Koča, DrSc.
- Other MU Faculty/Unit
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Central European Institute of Technology
- Keywords
- proteins, structure function relationships, protein complexes, proteome, metabolome
The proposal is the follow up of the previous, in our view successful, long-term research plan "Structure-Function Relationships of Biomolecules and their Role in Metabolism" studied in 1998-2004, and partly the project "Laboratory of Structure and Dynamics of Biomolecules" in the framework of program "250" studied in 1996-2000. The results of these projects were published in tens of scientific articles communicated in renowned international journals with high impact factors. The key aim was identification and characterization of low molecular weight substances involved in metabolism of bacteria and fungi with the special attention devoted to biochemical activities and the structure.
This new proposal will deal with other proteins from various organisms, for example animal proteins binding pheromones or proteins interacting with sacharides. The function and regulation of enzymes will be studied by novel methods suitable for monitoring of in vivo activity via monitoring of intracellular metabolites. We will continue to use structural methods. Keywords of the long-term research plan are protein, its function, structure, structure-function relationships and complexes of proteins. More general keywords are proteome and metabolome. The project will focus on such hot topics of current life sciences like protein/DNA interactions, synthesis of proteins on ribosome or applications of photo-labile groups for directed geometrical changes of protein-substrate complexes and regulation of enzymatic reactions.
Publications
Total number of publications: 835
2011
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Purinergic signaling in astrocytes: Induction of iodothyronine deiodinases enzyme activities
Year: 2011, type: Conference abstract
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QSPR modeling – algorithms, challenges and IT solutions
Year: 2011, type: Conference abstract
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Radioanalytical methods: Tools in studies of thyrotoxic effects of excessive bromide and perchlorate ions
Year: 2011, type: Conference abstract
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Radiometric determination of enzyme activities involved in biotransformations of thyroid hormones.
Year: 2011, type: Conference abstract
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Radiometric enzyme assays for iodothyronine deiodinases
Year: 2011, type: Conference abstract
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Radiometric enzyme assays: Extremely sensitive determination of iodothyronine deiodinases
Year: 2011, type: Conference abstract
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Radiopharmacological studies: Interaction of antidepressant Fluoxetine with thyroid hormones metabolism
Year: 2011, type: Conference abstract
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Random mutagenesis of lectins and high-throughput screening of mutant libraries
Year: 2011, type: Conference abstract
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Reactants Mixing inside the In-Capillary Nanoreactor: Utilization of Longitudinal and Transverse Diffusion
Year: 2011, type:
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Reaction Mechanism of MutH Enzyme - Quantum Mechanics/Molecular Mechanics Study 2
Year: 2011, type: Conference abstract