Project information
Proteins in metabolism and interaction of organisms with the environment
- Project Identification
- MSM0021622413
- Project Period
- 1/2005 - 12/2011
- Investor / Pogramme / Project type
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Ministry of Education, Youth and Sports of the CR
- Research Intents
- MU Faculty or unit
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Faculty of Science
- prof. RNDr. Jaroslav Koča, DrSc.
- Other MU Faculty/Unit
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Central European Institute of Technology
- Keywords
- proteins, structure function relationships, protein complexes, proteome, metabolome
The proposal is the follow up of the previous, in our view successful, long-term research plan "Structure-Function Relationships of Biomolecules and their Role in Metabolism" studied in 1998-2004, and partly the project "Laboratory of Structure and Dynamics of Biomolecules" in the framework of program "250" studied in 1996-2000. The results of these projects were published in tens of scientific articles communicated in renowned international journals with high impact factors. The key aim was identification and characterization of low molecular weight substances involved in metabolism of bacteria and fungi with the special attention devoted to biochemical activities and the structure.
This new proposal will deal with other proteins from various organisms, for example animal proteins binding pheromones or proteins interacting with sacharides. The function and regulation of enzymes will be studied by novel methods suitable for monitoring of in vivo activity via monitoring of intracellular metabolites. We will continue to use structural methods. Keywords of the long-term research plan are protein, its function, structure, structure-function relationships and complexes of proteins. More general keywords are proteome and metabolome. The project will focus on such hot topics of current life sciences like protein/DNA interactions, synthesis of proteins on ribosome or applications of photo-labile groups for directed geometrical changes of protein-substrate complexes and regulation of enzymatic reactions.
Publications
Total number of publications: 835
2010
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Radioiodine tracers as useful tools in studies of thyrotoxic effects of bromide and perchlorate ions
Year: 2010, type: Conference abstract
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Radiometric enzyme assays: Development of methods for extremely sensitive determination of types 1, 2 and 3 iodothyronine deiodinase enzyme activities
Year: 2010, type: Conference abstract
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Radiometric enzyme assays: Development of methods for extremely sensitive determination of types 1, 2 and 3 iodothyronine deiodinase enzyme activities
Chemické Listy, year: 2010, volume: 104, edition: 14, Spec.
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Radiometric determination of enzyme activities, exemplified by several key enzymes of thyroid hormone metabolism
Year: 2010, type: Conference abstract
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Radiometric determination of enzyme activities, exemplified by several key enzymes of thyroid hormone metabolism
Chemické Listy, year: 2010, volume: 104, edition: 14, Spec.
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Radiometric enzyme assays: development of methods for extremely sensitive determination of types 1, 2 and 3 iodothyronine deiodinase enzyme activities
Journal of Radioanalytical and Nuclear Chemistry, year: 2010, volume: 286, edition: 3
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Random mutagenesis as a tool for improving properties of PA-IIL lectin from Pseudomonas aeruginosa
Year: 2010, type: Conference abstract
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Reaction Mechanism of MutH Enzyme: Quantum Mechanics/Molecular Mechanics Study
Year: 2010, type: Conference abstract
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Recognition of selected monosaccharides by Pseudomonas aeruginosa Lectin II analyzed by molecular dynamics and free energy calculations
Carbohydrate Research, year: 2010, volume: 345, edition: 10
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Reference MP2/CBS and CCSD(T) quantum-chemical calculations on stacked adenine dimers. Comparison with DFT-D, MP2.5, SCS(MI)-MP2, M06-2X, CBS(SCS-D) and force field descriptions.
Physical Chemistry Chemical Physics, year: 2010, volume: 14, edition: -