Project information
Proteins in metabolism and interaction of organisms with the environment
- Project Identification
- MSM0021622413
- Project Period
- 1/2005 - 12/2011
- Investor / Pogramme / Project type
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Ministry of Education, Youth and Sports of the CR
- Research Intents
- MU Faculty or unit
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Faculty of Science
- prof. RNDr. Jaroslav Koča, DrSc.
- Other MU Faculty/Unit
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Central European Institute of Technology
- Keywords
- proteins, structure function relationships, protein complexes, proteome, metabolome
The proposal is the follow up of the previous, in our view successful, long-term research plan "Structure-Function Relationships of Biomolecules and their Role in Metabolism" studied in 1998-2004, and partly the project "Laboratory of Structure and Dynamics of Biomolecules" in the framework of program "250" studied in 1996-2000. The results of these projects were published in tens of scientific articles communicated in renowned international journals with high impact factors. The key aim was identification and characterization of low molecular weight substances involved in metabolism of bacteria and fungi with the special attention devoted to biochemical activities and the structure.
This new proposal will deal with other proteins from various organisms, for example animal proteins binding pheromones or proteins interacting with sacharides. The function and regulation of enzymes will be studied by novel methods suitable for monitoring of in vivo activity via monitoring of intracellular metabolites. We will continue to use structural methods. Keywords of the long-term research plan are protein, its function, structure, structure-function relationships and complexes of proteins. More general keywords are proteome and metabolome. The project will focus on such hot topics of current life sciences like protein/DNA interactions, synthesis of proteins on ribosome or applications of photo-labile groups for directed geometrical changes of protein-substrate complexes and regulation of enzymatic reactions.
Publications
Total number of publications: 835
2010
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Methods of directed evolution of PA-IIL lectin
Year: 2010, type: Conference abstract
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Microbial targeted metabolome analysis of common nucleotides and coenzymes by capillary electrophoresis
Final Program and Book of Abstracts, year: 2010
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Mobilization and phytotoxicity of iron under conditions of bacterial oxidation of iron sulfides
Year: 2010, type: Conference abstract
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Modulation of type I iodothyronine 5´-deiodinase activity in white adipose tissue by nutrition: Possible involvement of leptin
Physiological Research, year: 2010, volume: 59, edition: 4
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Molecular dynamics simulations suggest that RNA three-way junctions can act as flexible RNA structural elements in the ribosome
Nucleic Acids Research, year: 2010, volume: 38, edition: 18
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Monitoring of Cytochrome P450 2C9 isoform reaction with Diclofenac by In-Capillary System based on Transverse Diffusion of Laminar Flow Profiles
Book of Abtracts 16th Latin-American Symposium LACE 2010, year: 2010
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NMR as a tool for studies of (partially) disordered proteins and their interactions
Year: 2010, type: Conference abstract
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Novel lectins from Aspergillus fumigatus
Year: 2010, type: Conference abstract
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On the Role of the cis Hoogsteen:Sugar-Edge Family of Base Pairs in Platforms and Triplets: Quantum Chemical Insights into RNA Structural Biology
Jouranl of Physical Chemistry B, year: 2010, volume: 114, edition: -
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On-line drug metabolites generation and their subsequent target analysis by capillary zone electrophoresis with UV-absorption detection
Electrophoresis, year: 2010, volume: 31, edition: 19