Project information
Proteins in metabolism and interaction of organisms with the environment
- Project Identification
- MSM0021622413
- Project Period
- 1/2005 - 12/2011
- Investor / Pogramme / Project type
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Ministry of Education, Youth and Sports of the CR
- Research Intents
- MU Faculty or unit
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Faculty of Science
- prof. RNDr. Jaroslav Koča, DrSc.
- Other MU Faculty/Unit
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Central European Institute of Technology
- Keywords
- proteins, structure function relationships, protein complexes, proteome, metabolome
The proposal is the follow up of the previous, in our view successful, long-term research plan "Structure-Function Relationships of Biomolecules and their Role in Metabolism" studied in 1998-2004, and partly the project "Laboratory of Structure and Dynamics of Biomolecules" in the framework of program "250" studied in 1996-2000. The results of these projects were published in tens of scientific articles communicated in renowned international journals with high impact factors. The key aim was identification and characterization of low molecular weight substances involved in metabolism of bacteria and fungi with the special attention devoted to biochemical activities and the structure.
This new proposal will deal with other proteins from various organisms, for example animal proteins binding pheromones or proteins interacting with sacharides. The function and regulation of enzymes will be studied by novel methods suitable for monitoring of in vivo activity via monitoring of intracellular metabolites. We will continue to use structural methods. Keywords of the long-term research plan are protein, its function, structure, structure-function relationships and complexes of proteins. More general keywords are proteome and metabolome. The project will focus on such hot topics of current life sciences like protein/DNA interactions, synthesis of proteins on ribosome or applications of photo-labile groups for directed geometrical changes of protein-substrate complexes and regulation of enzymatic reactions.
Publications
Total number of publications: 835
2008
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NMR study of a single-stranded DNA binding to the C-terminal domain of the protease from murine intracisternal A-type particle
Year: 2008, type: Conference abstract
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Novel radiometric enzyme assays: Extremely sensitive determination (in 10 exp-18 to 10 exp-12 katals range) of iodothyronine deiodinases activities
Proceedings, 2nd International Nuclear Chemistry Congress (M. Navarrete, ed.), Rp 23, year: 2008, number of pages: 2 s.
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Nový protein rhodanasového typu u Acidithiobacillus ferrooxidans - funkční studie
Chem. Listy 102, 365–404 (2008) - VIII. Mezioborové setkání mladých biologů, biochemiků a chemiků, year: 2008
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On-capillary microreaction electrophoretic technique utilizing microsomes as an enzymatic system in drug metabolism studies
XII. Setkání biochemiků a molekulárních biologů, year: 2008
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On-line preconcentration of 4-hydroxydiclofenac for MEKC separation by means of sweeping in homogenous electric field
Book of Abstracts CECE 2008, year: 2008
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Optimalizace exprese a purifikace rekombinantního lektinu CV-IIL z patogenního mikroorganismu Chromobacterium violaceum
Študentská vedecká konferencia 23.apríl 2008, Zborník prispevkov, 1.zväzok, biologická sekcia, year: 2008
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Pathways and Mechanisms for Product Exit and Water Exchange in Engineered Haloalkane Dehalogenase DhaA Exploredusing Classical and Random Acceleration Molecular Dynamics Simulations
Year: 2008, type: R&D Presentation
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Photochemical Synthesis of Substituted Indan-1-ones Related to Donepezil
Photochemical & Photobiological Sciences, year: 2008, volume: 7, edition: 5
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Photoinitiated polymerisation of monolithic stationary phases in polyimide coated capillaries using visible region LEDs
Chemical Communications, year: 2008, volume: 48, edition: 28
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Porucha barvocitu u alkoholiků a její souvislost s polymorfizmem genu pro BDNF
Česká a slovenská psychiatrie, year: 2008, volume: 104, edition: 3