Project information
Proteins in metabolism and interaction of organisms with the environment
- Project Identification
- MSM0021622413
- Project Period
- 1/2005 - 12/2011
- Investor / Pogramme / Project type
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Ministry of Education, Youth and Sports of the CR
- Research Intents
- MU Faculty or unit
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Faculty of Science
- prof. RNDr. Jaroslav Koča, DrSc.
- Other MU Faculty/Unit
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Central European Institute of Technology
- Keywords
- proteins, structure function relationships, protein complexes, proteome, metabolome
The proposal is the follow up of the previous, in our view successful, long-term research plan "Structure-Function Relationships of Biomolecules and their Role in Metabolism" studied in 1998-2004, and partly the project "Laboratory of Structure and Dynamics of Biomolecules" in the framework of program "250" studied in 1996-2000. The results of these projects were published in tens of scientific articles communicated in renowned international journals with high impact factors. The key aim was identification and characterization of low molecular weight substances involved in metabolism of bacteria and fungi with the special attention devoted to biochemical activities and the structure.
This new proposal will deal with other proteins from various organisms, for example animal proteins binding pheromones or proteins interacting with sacharides. The function and regulation of enzymes will be studied by novel methods suitable for monitoring of in vivo activity via monitoring of intracellular metabolites. We will continue to use structural methods. Keywords of the long-term research plan are protein, its function, structure, structure-function relationships and complexes of proteins. More general keywords are proteome and metabolome. The project will focus on such hot topics of current life sciences like protein/DNA interactions, synthesis of proteins on ribosome or applications of photo-labile groups for directed geometrical changes of protein-substrate complexes and regulation of enzymatic reactions.
Publications
Total number of publications: 835
2006
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The effect of chemical shift tensor asymmetry on relaxation behavior of base carbons in nucleic acid
21th NMR Valtice, year: 2006
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The gene for interleukin 6: the candidate gene for neuropsychiatric illness?
American Journal of Medical Genetics, year: 2006, volume: 141B, edition: 7
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The Identification of Catalytic Pentad in the Haloalkane Dehalogenase DhmA from Mycobacterium avium N85: Reaction Mechanism and Molecular Evolution
JOURNAL OF STRUCTURAL BIOLOGY, year: 2006, volume: 1/2006, edition: 1
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The studies of tautomerism in 6-mercaptopurine derivatives by 1H-13C, 1H-15N NMR and 13C, 15N CP/MAS-experimental and quantum chemical approach
Journal of Molecular Structure, year: 2006, volume: 785, edition: 1
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Theoretical Study of the Effects of DNA Stacking Interactions on the NMR Chemical Shifts
21th NMR Valtice, year: 2006
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Trapped water molecules are essential to structural dynamics and function of a ribozyme
The Proceedings of the National Academy of Sciences, year: 2006, volume: 103, edition: 36
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Výhody a úskalí produkce proteinů v expresním systému kvasinky Pichia pastoris
Year: 2006, type: Conference abstract
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Využití MALDI-MS pro identifikaci Pseudomonas spp.
Year: 2006, type: Conference abstract
2005
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13C-Detected Experiments for NMR Spectroscopy of Nucleic Acids
EUROMAR/EENC 2005, year: 2005
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13C-Detected Experiments for Nucleic Acids
20th NMR Valtice, year: 2005