Project information
Single cell analysis: a modern tool to study clonal evolution in high-risk patients with chronic lymphocytic leukemia
(Single cell analysis of CLL cells)
- Project Identification
- NU20-08-00314
- Project Period
- 5/2020 - 12/2023
- Investor / Pogramme / Project type
-
Ministry of Health of the CR
- Ministry of Health Research Programme 2020 - 2026
- Subprogram 1 - standard
- MU Faculty or unit
- Central European Institute of Technology
- Cooperating Organization
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University Hospital Brno-Bohunice
- Responsible person Mgr. Jana Kotašková, Ph.D.
The proposed project builds on continuous well established research of our team in the field of hematologic malignancies. In this study we would like to profound our knowledge and understanding the mechanisms underlying clonal evolution of chronic lymphocytic leukaemia (CLL) cells. We have already studied CLL using variety of bulk analysis moreover we have also succeeded in single cell analysis. We believe that true personalised medicine is based of detailed characterisation of malignant cells of each patient with the potential to detect the most aggressive clones. From this prospective, single cell analysis gives the best opportunity to fulfil this need. The aim of our study is to compare transcriptomes of thousands CLL cells from consecutively collected samples of patients with aggressive disease with the aim to define the properties of subclones responsible for disease refractoriness.
Sustainable Development Goals
Masaryk University is committed to the UN Sustainable Development Goals, which aim to improve the conditions and quality of life on our planet by 2030.
Publications
Total number of publications: 20
2024
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Integrative NGS testing reveals clonal dynamics of adverse genomic defects contributing to a natural progression in treatment-naïve CLL patients
British journal of haematology, year: 2024, volume: 204, edition: 1, DOI
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IκBε deficiency accelerates disease development in chronic lymphocytic leukemia
Leukemia, year: 2024, DOI
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Unveiling the dynamics and molecular landscape of a rare chronic lymphocytic leukemia subpopulation driving refractoriness: insights from single-cell RNA sequencing
Molecular oncology, year: 2024, DOI
2023
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A web-based bioinformatic tool LYNX for targeted LYmphoid NeXt- generation sequencing data analysis and visualization for hematooncology.
Year: 2023, type: Conference abstract
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Cerebral organoids derived from patients with Alzheimer´s disease with PSEN1/2 mutations have defective tissue patterning and altered development
CELL REPORTS, year: 2023, volume: 42, edition: 11, DOI
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RNA sekvenovanie jednotlivých buniek umožňuje charakterizáciu a sledovanie zriedkavých CLL buniek s potenciálom navodiť refraktérne ochorenie.
Year: 2023, type: Conference abstract
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Single-cell RNA-sequencing enables tracking and characterization of rare CLL cells with the potential to cause refractoriness.
Year: 2023, type: Conference abstract
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Subclonal architecture of chromosomes revealed by single-cell analysis of gene expression in a patient with clonal evolution of relapsing/refractory CLL.
Year: 2023, type: Conference abstract
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Tracking CLL cells with aberrations in the TP53 gene using scRNA-seq in relapsed/refractory patients.
Year: 2023, type: Conference abstract
2022
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Cytogenetics in Chronic Lymphocytic Leukemia: ERIC Perspectives and Recommendations
HemaSphere, year: 2022, volume: 6, edition: 4, DOI