Project information
Single cell analysis: a modern tool to study clonal evolution in high-risk patients with chronic lymphocytic leukemia
(Single cell analysis of CLL cells)
- Project Identification
- NU20-08-00314
- Project Period
- 5/2020 - 12/2023
- Investor / Pogramme / Project type
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Ministry of Health of the CR
- Ministry of Health Research Programme 2020 - 2026
- Subprogram 1 - standard
- MU Faculty or unit
- Central European Institute of Technology
- Cooperating Organization
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University Hospital Brno-Bohunice
- Responsible person Mgr. Jana Kotašková, Ph.D.
The proposed project builds on continuous well established research of our team in the field of hematologic malignancies. In this study we would like to profound our knowledge and understanding the mechanisms underlying clonal evolution of chronic lymphocytic leukaemia (CLL) cells. We have already studied CLL using variety of bulk analysis moreover we have also succeeded in single cell analysis. We believe that true personalised medicine is based of detailed characterisation of malignant cells of each patient with the potential to detect the most aggressive clones. From this prospective, single cell analysis gives the best opportunity to fulfil this need. The aim of our study is to compare transcriptomes of thousands CLL cells from consecutively collected samples of patients with aggressive disease with the aim to define the properties of subclones responsible for disease refractoriness.
Sustainable Development Goals
Masaryk University is committed to the UN Sustainable Development Goals, which aim to improve the conditions and quality of life on our planet by 2030.
Publications
Total number of publications: 19
2022
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Gene expression analysis on single cell level uncovers the subclonal architecture of chromosomes in a leukemia patient with multiple chromosomal aberrations.
Year: 2022, type: Conference abstract
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Interrogating the molecular heterogeneity of chronic lymphocytic leukemia through computational approaches.
Year: 2022, type: Conference abstract
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Přínos a úskalí sekvenačních metod v diagnostice hematologických malignit.
Year: 2022, type: Conference abstract
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Realita zapojení molekulárně biologických metod do diagnostiky a stratifikace lymfoproliferativních onemocnění.
Year: 2022, type: Conference abstract
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Single-cell RNA sequencing analysis of T helper cell differentiation and heterogeneity
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, year: 2022, volume: 1869, edition: 10, DOI
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Současná role cytogenomiky v diagnostice a prognostické stratifikaci hematologických malignit.
Year: 2022, type: Conference abstract
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Subclonal architectureof chromosomes revealed by single-cell analysis of gene expression in a patient with clonal evolution of relapsing/refractory CLL
Year: 2022, type: Conference abstract
2021
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B cell developmental trajectory modeling – Using complex immunophenotype to identify pre-malignant CLL B cell subsets in peripheral blood of healthy donors.
Year: 2021, type: Conference abstract
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Data mining of publicly available scRNA-seq datasets: application of machine learning to interrogate normal cellular counterparts of chronic lymphocytic leukemia.
Year: 2021, type: Conference abstract