Project information
Changes of the cytoskeleton in tumour cell lines during induced apoptosis in relation to different stages of differentiation
- Project Identification
- GA304/00/0403
- Project Period
- 1/2000 - 1/2002
- Investor / Pogramme / Project type
-
Czech Science Foundation
- Standard Projects
- MU Faculty or unit
- Faculty of Medicine
Apoptosis, a programmed cell death, is worth of study not only as aform of physiological cell death but also as a promising approach to the casual theraphy of malignant diseases. In spite of great progress in this field is not well understood how the apo ptosis is modulated in differentiated cells and how cytoskeletal components are involved. In this project a detailed study of the cytoskeletal components in two tumour cell lines is proposed with the aim to define a role of the cytoskeleton in the trigge ring and the course of apoptosis. Human promyelocytic leukemia HL-60 and mouse embryonal carcinoma P19 cell lines will be used as well as their differentiated forms-monocytes, granulocytes and macrophages (HL-60 cells) and neural, glial and muscle cells (P19 cells). The apoptosis will be triggered by chemical inductors including cytoskeletal toxins and different techniques will be applied to describe the process at the molecular (SDS-PAGE, immunoblotting), microscopical (immunofluorescence) and ultrastr
Publications
Total number of publications: 28
2002
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Caffeic acid affects an induced differentiation process into granulocyte stage in HL-60 myeloid leukaemia cell line
Cells IV, year: 2002
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Changes in the cytoskeleton of HL-60 and P19 cells during apoptosis associated with differentiation process.
Year: 2002, type: Conference abstract
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Changes in the cytoskeleton of P19 cells during apoptosis associated with induced differentiation
Cells IV, year: 2002
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Induced differentiation and apoptosis in HL-60 cell line modulated by caffeic acid treatment
Year: 2002, type: Conference abstract
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The role of actin cytoskeleton in apoptotic cell death
Year: 2002, type: Conference abstract
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The role of actin in the apoptotic cell death
Year: 2002, type: Conference abstract
2001
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Cytoskelet buněk myeloidní leukemické linie HL-60 ve vztahu k indukci apoptózy a diferenciace
Year: 2001, type: Conference abstract
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Effect of retinoic acid on the actin cytoskeleton in HL-60 cells
Neoplasma, year: 2001, volume: 48, edition: 6
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Changes in the cytoskeleton during apoptotic cell death
Year: 2001, type: Conference abstract
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Studie protektivního vlivu alfa-tokoferolu a kyseliny kávové na cytoskelet diploidních a transformovaných lidských buněk in vitro po ozáření UV-C
Year: 2001, type: Conference abstract