Project information
Delta podjednotka RNA polymerázy z Gram pozitivních bakterií
(DELTA)
- Project Identification
- GA204/09/0583
- Project Period
- 1/2009 - 12/2012
- Investor / Pogramme / Project type
-
Czech Science Foundation
- Standard Projects
- MU Faculty or unit
- Central European Institute of Technology
- Keywords
- RNA polymerase
- Cooperating Organization
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Institute of Microbiology of the ASCR, v. v. i.
- Responsible person Mgr. Libor Krásný, Ph.D.
To efficiently fight bacterial diseases and infections, knowledge of the molecular mechanisms used by these organisms is required. Bacterial RNA polymerase (RNAP) is an essential multisubunit enzyme. Contrary to the well studied RNAPs from gram negative bacteria, gram positive RNAPs contain the d subunit. Its role in the cell is not well understood. We propose to conduct structural, genetic, and biochemical studies to define its role in gene expression regulation. We propose to solve the 3D structure of delta, determine its position within RNAP, and use this new knowledge to define its physiological role in the cell.
Publications
Total number of publications: 19
2013
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Structural Study of the Partially Disordered Full-Length delta Subunit of RNA Polymerase from Bacillus subtilis
ChemBioChem, year: 2013, volume: 14, edition: 14, DOI
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The Delta Subunit of RNA Polymerase Is Required for Rapid Changes in Gene Expression and Competitive Fitness of the Cell
Journal of Bacteriology, year: 2013, volume: 195, edition: 11, DOI
2012
2011
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5D 13C-detected experiments for backbone assignment of unstructured proteins with a very low signal dispersion
Journal of Biomolecular NMR, year: 2011, volume: 50, edition: 1, DOI
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Complementation of 3D structure of delta subunit of RNA polymerase from Bacillus subtilis with description of internal motions in terms of reduced spectral density mapping
MATERIALS STRUCTURE, year: 2011, volume: 18, edition: 1
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Crystallization and structure determination of delta-subunit of RNApolymerase
Year: 2011, type: Conference abstract
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NMR study of an RNA polymerase subunit unique for gram-positive bacteria
Year: 2011, type: Appeared in Conference without Proceedings
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Structural analysis of delta-subnit of RNA-polymerase by X-ray
Year: 2011, type: Conference abstract
-
Study of RNA polymerase delta subunit unique for gram-positive bacteria
Year: 2011, type: Conference abstract
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The X-ray structure versus NMR structure of delta-subunit of RNA-polymerase
Year: 2011, type: Conference abstract