Project information
Delta podjednotka RNA polymerázy z Gram pozitivních bakterií
(DELTA)
- Project Identification
- GA204/09/0583
- Project Period
- 1/2009 - 12/2012
- Investor / Pogramme / Project type
-
Czech Science Foundation
- Standard Projects
- MU Faculty or unit
- Central European Institute of Technology
- Keywords
- RNA polymerase
- Cooperating Organization
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Institute of Microbiology of the ASCR, v. v. i.
- Responsible person Mgr. Libor Krásný, Ph.D.
To efficiently fight bacterial diseases and infections, knowledge of the molecular mechanisms used by these organisms is required. Bacterial RNA polymerase (RNAP) is an essential multisubunit enzyme. Contrary to the well studied RNAPs from gram negative bacteria, gram positive RNAPs contain the d subunit. Its role in the cell is not well understood. We propose to conduct structural, genetic, and biochemical studies to define its role in gene expression regulation. We propose to solve the 3D structure of delta, determine its position within RNAP, and use this new knowledge to define its physiological role in the cell.
Publications
Total number of publications: 19
2010
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NMR as a tool for studies of (partially) disordered proteins and their interactions
Year: 2010, type: Conference abstract
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S3EPY: a Sparky extension for determination of small scalar couplings from spin-state-selective excitation NMR experiments
Journal of Biomolecular NMR, year: 2010, volume: 46, edition: 2
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Solution structure of the N-terminal domain of Bacillus subtilis delta subunit of RNA polymerase and its classification based on structural homologs
Proteins: Structure, Function, and Bioinformatics, year: 2010, volume: 78, edition: 7
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Strategy for complete NMR assignment of disordered proteins with highly repetitive sequences based on resolution-enhanced 5D experiments
Journal of Biomolecular NMR, year: 2010, volume: 48, edition: 3
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Structure and dynamics of an RNA polymerase subunit unique for gram-positive bacteria
Year: 2010, type: Conference abstract
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Structure and dynamics of RNA polymerase delta subunit from Bacillus subtilis determined by NMR spectroscopy
Year: 2010, type: Conference abstract
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Structure determination of an RNA polymerase subunit unique for gram-positive bacteria
Year: 2010, type: Conference abstract
2009
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NMR structural study of N-terminal domain of RNA polymerase delta subunit from Bacillus subtilis
Year: 2009, type: Conference abstract
-
Structural study of an RNA polymerase subunit unique for gram-positive bacteria
Year: 2009, type: Conference abstract