Publication details
Immunomagnetic separation in the detection of Salmonella cells from foodstuffs
| Authors | |
|---|---|
| Year of publication | 1999 |
| Type | Article in Proceedings |
| Conference | 13th Internal Symposium of Affinity Technology and Bio-Recognition |
| MU Faculty or unit | |
| Citation | |
| Field | Biotechnology |
| Description | Cultivation method and polymerase chain reaction coupled with immunomagnetic separation (IMS-CM and IMS-PCR) are new technoques used for the detection of bacteria in foodstuffs. In this study, Salmonella was isolated from various real food samples by magnetic Dynabeads M-280 anti-Salmonella using both IMS-CM and IMS-PCR. Food samples included (milk, eggs, meat products, confectionery, vegetable, fruit products) and total 408 specimens were investigated. 49 samples (i.e. 12%) were found Salmonella positive by IMS-CM. Bacteria coupled on immunomagnetic particles were also used in IMS-PCR. Growing atypically on sensitive medium two Salmonella strains were identified using IMS-PCR. False negative results were obtained by conbventional technques. The above mentioned results demonstrated that IMS-CM and IMS-PCR techniques are more sensetive in real food samples analysis than conventional cultivation procedures. |
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