Publication details
Lokalizace spektrinu (spektrinu podobného) proteinu u kvasinek
| Title in English | Localisation of spectrin - like protein in yeast |
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| Authors | |
| Year of publication | 2001 |
| Type | Article in Proceedings |
| Conference | In IX. Cytoskeletální klub |
| MU Faculty or unit | |
| Citation | |
| Field | Morphological specializations and cytology |
| Keywords | spectrin; yeast |
| Description | Spectrin-like antigens were immunochemically detected and by immunofluorescence and/or immunogold-labelling localized in cells and protoplasts of budding yeasts Saccharomyces cerevisiae and fission yeasts Schizosaccharomyces japonicus var. versatilis. In these yeasts spectrin-like antigens were detected on Western blots at 220 kDa with anti-chicken and anti-human erythrocyte spectrin antibodies. Additional bands were present at 50 kDa in S. cerevisiae, 44 kDa in Sch. versatilis, 34 kDa and 20 kDa in both yeasts. Since at these molecular masses human erythrocyte spectrin was also detected, these bands could show breakdown products of spectrin or other spectrin-like proteins. Fluorescence microscope revealed labelling of spectrin-like antigens on the cell surface and vacuolar membrane and dotted fluorescence of cytoplasm of cells and protoplasts of both yeast species. In Sch. versatilis faint fluorescence of nuclei was observed. During sporulation and in mature spores spectrin-like proteins correspondently to actin patches bordered spore surface. Electron microscopy revealed immunogold staining of plasma membrane, ER, vacuole and nuclei of (in) protoplasts and cells and cell wall of cells. In higher eukaryotic cells spectrin seems to be actin-crosslinking protein (Fowler and Ruijter, 2000). Our experiments with Latrunculin B, act1-1 and cdc 42 mutants and osmotic shock indicate independence of actin and spectrin. These observations indicate the role of spectrin in stabilization of plasma membrane and other membrane structures in yeasts. |
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