Separation of PCR-ready DNA from dairy products using magnetic hydrophilic microspheres and poly(ethylene glykol)-NaCl water solutions

• Authors: RITTICH Bohuslav; ŠPANOVÁ Alena; ŠÁLEK Petr; NĚMCOVÁ Petra; TRACHTOVÁ Štěpánka
• Year of publication: 2009
• Type: Article in Periodical
• Magazine / Source: Journal of Magnetism and Magnetic Materials
• MU Faculty or unit: Faculty of Science
• Field: Genetics and molecular biology
• Keywords: Magnetic microsphere; P(HEMA-co-GMA); DNA isolation; dairy product; polymerase chain reaction (PCR)
• Description: Carboxyl group-containing magnetic nonporous poly(2-hydroxyethyl methacrylate-co- glycidyl dimethylmethacrylate) (P(HEMA-co-GMA)) and magnetic glass microspheres were used for the isolation of bacterial DNA. The most extensive DNA recovery achieved at PEG 6000 concentrations of 12% or 16 % and 2 M NaCl. The method proposed was used for bacterial DNA isolation from different dairy products containing Bifidobacterium and Lactobacillus cells. The presence of target DNA and quality of isolated DNA was checked by PCR amplification with specific primers.

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