Project information
Proteins in metabolism and interaction of organisms with the environment
- Project Identification
- MSM0021622413
- Project Period
- 1/2005 - 12/2011
- Investor / Pogramme / Project type
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Ministry of Education, Youth and Sports of the CR
- Research Intents
- MU Faculty or unit
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Faculty of Science
- prof. RNDr. Jaroslav Koča, DrSc.
- Other MU Faculty/Unit
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Central European Institute of Technology
- Keywords
- proteins, structure function relationships, protein complexes, proteome, metabolome
The proposal is the follow up of the previous, in our view successful, long-term research plan "Structure-Function Relationships of Biomolecules and their Role in Metabolism" studied in 1998-2004, and partly the project "Laboratory of Structure and Dynamics of Biomolecules" in the framework of program "250" studied in 1996-2000. The results of these projects were published in tens of scientific articles communicated in renowned international journals with high impact factors. The key aim was identification and characterization of low molecular weight substances involved in metabolism of bacteria and fungi with the special attention devoted to biochemical activities and the structure.
This new proposal will deal with other proteins from various organisms, for example animal proteins binding pheromones or proteins interacting with sacharides. The function and regulation of enzymes will be studied by novel methods suitable for monitoring of in vivo activity via monitoring of intracellular metabolites. We will continue to use structural methods. Keywords of the long-term research plan are protein, its function, structure, structure-function relationships and complexes of proteins. More general keywords are proteome and metabolome. The project will focus on such hot topics of current life sciences like protein/DNA interactions, synthesis of proteins on ribosome or applications of photo-labile groups for directed geometrical changes of protein-substrate complexes and regulation of enzymatic reactions.
Publications
Total number of publications: 835
2007
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Multichannel SPR biosensor for detection of endocrine-disrupting compounds
Analytical and Bioanalytical Chemistry, year: 2007, volume: 389, edition: 6
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Mutagenesis of PA-IIL and related affinity changes - molecular modeling in service of protein engineering
Konference Grantové Agentury České republiky - Strukturní biofyzika makromolekul II., year: 2007
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Nahlédnutí do struktury a funkce lektinu RS20L z bakteriálního fytopatogenu Ralstonia solanacearum
Chem. Listy 101, 425–465 (2007) - VII. Mezioborové setkání mladých biologů, biochemiků a chemiků, year: 2007
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Neurobiologie hyperkinetické poruchy
Česká a slovenská psychiatrie, year: 2007, volume: 103, edition: 1
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New approaches to structure and function studies of RS20L lectin from Ralstonia solanacearum
14th European Carbohydrate Symposium, EUROCARB 14, year: 2007
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New approaches to structure and function studies of RS20L lectin from Ralstonia solanacearum
Materials Structure, vol. 14, no. 1 (2007), 6th Discussions in Structural Molecular Biology, year: 2007
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Novel 13C-detected Experiments for Nucleic Acids
11th Chianti Workshop on Magnetic Resonance, year: 2007
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P-31 Chemical Shift Tensors in RNA and DNA Backbone. A DFT Study of Conformational Dependence and Its Implications for NMR Studies.
Year: 2007, type: Conference abstract
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Partially O-Alkylated thiacalix[4]arenes: Synthesis, molecular and crystal structures, conformational behavior
Journal of Organic Chemistry, year: 2007, volume: 72, edition: 19
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Photochromism of phenoxynaphthacenequinones: diabatic or adiabatic phenyl group transfer?
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, year: 2007, volume: 6/2007, edition: 5