Project information
Proteins in metabolism and interaction of organisms with the environment
- Project Identification
- MSM0021622413
- Project Period
- 1/2005 - 12/2011
- Investor / Pogramme / Project type
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Ministry of Education, Youth and Sports of the CR
- Research Intents
- MU Faculty or unit
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Faculty of Science
- prof. RNDr. Jaroslav Koča, DrSc.
- Other MU Faculty/Unit
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Central European Institute of Technology
- Keywords
- proteins, structure function relationships, protein complexes, proteome, metabolome
The proposal is the follow up of the previous, in our view successful, long-term research plan "Structure-Function Relationships of Biomolecules and their Role in Metabolism" studied in 1998-2004, and partly the project "Laboratory of Structure and Dynamics of Biomolecules" in the framework of program "250" studied in 1996-2000. The results of these projects were published in tens of scientific articles communicated in renowned international journals with high impact factors. The key aim was identification and characterization of low molecular weight substances involved in metabolism of bacteria and fungi with the special attention devoted to biochemical activities and the structure.
This new proposal will deal with other proteins from various organisms, for example animal proteins binding pheromones or proteins interacting with sacharides. The function and regulation of enzymes will be studied by novel methods suitable for monitoring of in vivo activity via monitoring of intracellular metabolites. We will continue to use structural methods. Keywords of the long-term research plan are protein, its function, structure, structure-function relationships and complexes of proteins. More general keywords are proteome and metabolome. The project will focus on such hot topics of current life sciences like protein/DNA interactions, synthesis of proteins on ribosome or applications of photo-labile groups for directed geometrical changes of protein-substrate complexes and regulation of enzymatic reactions.
Publications
Total number of publications: 835
2007
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METALO + PROTEIN = GE + LA + ICP + MS
Year: 2007, type: Conference abstract
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Micellar Electrokinetic Capillary Chromatography as a Tool for Cytochrome P450 2C9 Activity Study
HPLC 2007. Abstract Book., year: 2007
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Microsomes & Microreaction in CE (invited lecture)
Year: 2007, type: Appeared in Conference without Proceedings
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MOLE: A Voronoi Diagram-based Explorer of Molecular Channels, Pores and Tunnels
Structure, year: 2007, volume: 15, edition: 15
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Molecular dissection of Rad52 mediator activity
Year: 2007, type: R&D Presentation
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Molecular dynamics simulations of quadruplex DNA.
First international meeting on quadruplex DNA,, year: 2007
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Molecular dynamics simulations of RNA: An in silico single molecule approach
BIOPOLYMERS, year: 2007, volume: 85, edition: 2
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Molecular Dynamics Simulations on Cyclin Dependent Kinases 2 and 5. Contribution to Designing New Inhibitors and Understanding Mechanism of Activation/Inhibition
Modeling Interaction in Biomolecules III, year: 2007
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Molecular modeling in hands of protein engineers - the lectin-saccharide interaction study
FEBS Journal 274 (suppl. 1), 32th FEBS Congress "Molecular Machines", year: 2007
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Molecular understanding of DSB repair-Mediator proteins
Year: 2007, type: R&D Presentation