Project information
Proteins in metabolism and interaction of organisms with the environment
- Project Identification
- MSM0021622413
- Project Period
- 1/2005 - 12/2011
- Investor / Pogramme / Project type
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Ministry of Education, Youth and Sports of the CR
- Research Intents
- MU Faculty or unit
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Faculty of Science
- prof. RNDr. Jaroslav Koča, DrSc.
- Other MU Faculty/Unit
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Central European Institute of Technology
- Keywords
- proteins, structure function relationships, protein complexes, proteome, metabolome
The proposal is the follow up of the previous, in our view successful, long-term research plan "Structure-Function Relationships of Biomolecules and their Role in Metabolism" studied in 1998-2004, and partly the project "Laboratory of Structure and Dynamics of Biomolecules" in the framework of program "250" studied in 1996-2000. The results of these projects were published in tens of scientific articles communicated in renowned international journals with high impact factors. The key aim was identification and characterization of low molecular weight substances involved in metabolism of bacteria and fungi with the special attention devoted to biochemical activities and the structure.
This new proposal will deal with other proteins from various organisms, for example animal proteins binding pheromones or proteins interacting with sacharides. The function and regulation of enzymes will be studied by novel methods suitable for monitoring of in vivo activity via monitoring of intracellular metabolites. We will continue to use structural methods. Keywords of the long-term research plan are protein, its function, structure, structure-function relationships and complexes of proteins. More general keywords are proteome and metabolome. The project will focus on such hot topics of current life sciences like protein/DNA interactions, synthesis of proteins on ribosome or applications of photo-labile groups for directed geometrical changes of protein-substrate complexes and regulation of enzymatic reactions.
Publications
Total number of publications: 835
2007
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High affinity lectins from human opportunistic pathogens: structure/function studies
XIX International Symposium on Glycoconjugates, year: 2007
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Human telomeric G-DNA - a test example for force field adjustment
Journal Of Biomolecular Structure and Dynamics, year: 2007
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Identification of Armillaria species from soil by nested-PCR reaction
Communicationes Instituti Forestalis Bohemicae, year: 2007
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In silico and In vitro Protein Engineering as a Bioanalytical Tool
CECE 2007, year: 2007
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In vitro and in silico protein engineering of lectins
14th European Carbohydrate Symposium, EUROCARB 14, year: 2007
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Integration of short-end injection procedure into EMMA methodology.
Journal of Chromatography A, year: 2007, volume: 1155, edition: 1-2
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Interesting behaviour of sugar-binding protein from phytopathogen Rasltonia solanacearum
Konference Grantové Agentury České republiky, year: 2007
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Kinetic constant variability in bacterial oxidation of elemental sulfur
Applied and Environmental Microbiology, year: 2007, volume: 73, edition: 11
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Limits of Accuracy of RNA Dynamics Studied by Model Free Analysis of C-13 Relaxation
22nd NMR Valtice, year: 2007
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Mannose-specific lectin from Burkholderia cenocepacia - structure and binding properties
International Congress of Young Chemists YoungChem 2007, year: 2007