Project information
Proteins in metabolism and interaction of organisms with the environment
- Project Identification
- MSM0021622413
- Project Period
- 1/2005 - 12/2011
- Investor / Pogramme / Project type
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Ministry of Education, Youth and Sports of the CR
- Research Intents
- MU Faculty or unit
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Faculty of Science
- prof. RNDr. Jaroslav Koča, DrSc.
- Other MU Faculty/Unit
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Central European Institute of Technology
- Keywords
- proteins, structure function relationships, protein complexes, proteome, metabolome
The proposal is the follow up of the previous, in our view successful, long-term research plan "Structure-Function Relationships of Biomolecules and their Role in Metabolism" studied in 1998-2004, and partly the project "Laboratory of Structure and Dynamics of Biomolecules" in the framework of program "250" studied in 1996-2000. The results of these projects were published in tens of scientific articles communicated in renowned international journals with high impact factors. The key aim was identification and characterization of low molecular weight substances involved in metabolism of bacteria and fungi with the special attention devoted to biochemical activities and the structure.
This new proposal will deal with other proteins from various organisms, for example animal proteins binding pheromones or proteins interacting with sacharides. The function and regulation of enzymes will be studied by novel methods suitable for monitoring of in vivo activity via monitoring of intracellular metabolites. We will continue to use structural methods. Keywords of the long-term research plan are protein, its function, structure, structure-function relationships and complexes of proteins. More general keywords are proteome and metabolome. The project will focus on such hot topics of current life sciences like protein/DNA interactions, synthesis of proteins on ribosome or applications of photo-labile groups for directed geometrical changes of protein-substrate complexes and regulation of enzymatic reactions.
Publications
Total number of publications: 835
2006
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Modification of Trypsin for Improvement of Its Stability in Protein Digestion Process
Year: 2006, type: Conference abstract
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Molecular biology of ADHD: an association study of DAT1, DRD2, DRD4, COMT and MAOB gene polymorphisms with hyperactive disorder in children
3rd International Biology Students Conference 2006, April 6.-9. 2006, year: 2006
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Molecular dynamics simulations of nucleic acids: MD simulations of G-DNA and functional RNAs
Challenges and advances in computational chemistry and physics. Computational studies of RNA and DNA, year: 2006, number of pages: 26 s.
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Molecular dynamics simulations of sarcin-ricin rRNA motif
Nucleic Acids Research, year: 2006, volume: 34, edition: 2
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Molecular dynamics study of major urinary protein-pheromone interactions: A structural model for ligand-induced flexibility increase
FEBS Letters, year: 2006, volume: 580, edition: 1
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Molecular interactions in nucleic acids
Senior research fellows' meeting, year: 2006
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Molecular modelling of glycosyltransferases
Glycobiology Protocols, year: 2006, number of pages: 12 s.
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Mycobacteria as a Source of Haloalkane Dehalogenases: Identification of the Catalytic Pentad within the Structure of DhmA from Mycobacterium avium N85.
Year: 2006, type: R&D Presentation
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NMR Distance Restraints At Low Temperatures: Application To DNA Hairpin
International school on High field NMR spectroscopy for solids and liquids, year: 2006
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NMR experiments for detection of quaternary carbons in nucleic acid bases.
8th Central European NMR Symposium, year: 2006