Project information
Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies
- Project Identification
- MSM0021622430
- Project Period
- 1/2007 - 12/2013
- Investor / Pogramme / Project type
-
Ministry of Education, Youth and Sports of the CR
- Research Intents
- MU Faculty or unit
- Faculty of Medicine
- Other MU Faculty/Unit
- Faculty of Science
- Other MU Faculty/Unit
- Faculty of Informatics
- Other MU Faculty/Unit
- Central European Institute of Technology
- Keywords
- embryonic stem cells
The goal of this project will be a) to profit from availability of unique cellular resources - mouse and human embryonic stem cells and normal and malignant hematopoietic stem/progenitor cells, b) to effectively employ a wide variety of current and complementary methodologies available to the consortium, and c) to exploit a proven/certified expertise in working with various living systems that span from in vitro cultured cells, through animal models to oncohematological patients. All this together will represent a powerful experimental platform aimed at addressing a nature of molecules and molecular mechanisms underlaying phenomena that are common to both normal and cancer stem cells, mainly their ability to self-renew and to enter differentiation pathways. Integral part of this general goal is to implement multifaceted analytical approach that will cover studies of gene expression at both mRNA and protein levels, evaluating function of extra and intracellular signaling pathways, and analyzing biological properties of cells in vitro and in vivo. Expected outcome will be not only understanding but also a definition of molecules and/or molecular pathways to be tested as therapeutic targets, and cellular models that will make such testing feasible.
Publications
Total number of publications: 358
2009
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Detection and treatment of molecular relapse in acute myeloid leukemia with RUNX1 (AML1), CBFB, or MLL gene translocations: Frequent quantitative monitoring of molecular markers in different compartments and correlation with WT1 gene expression
Experimental Hematology, year: 2009, volume: 37, edition: 6, DOI
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Detekce mutace JAK2 V617F u myeloproliferativních onemocnění - přehled a vlastní zkušenosti
Transfuze a hematologie dnes, year: 2009, volume: 15, edition: 3
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Efficacious but insidious: A retrospective analysis of fludarabine induced myelotoxicity using long term culture initiating cells in 100 follicular lymphoma patients
Experimental Hematology, year: 2009, volume: 37, edition: 11
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Focused microarray for lymphoma diagnostics.
Year: 2009, type: Conference abstract
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Gene-expression profiling of graft-versus-host disease by oligonucleotide microarrays
Year: 2009, type: Conference abstract
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Hematopoietic cells CD133+ and CD34+ selected and cultivated from bone marrow of leukemic patients.
Morphology 2009, year: 2009
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High molecular weight FGF2: the biology of a nuclear growth factor
Cellular and molecular life sciences : CMLS, year: 2009, volume: 66, edition: 2, DOI
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Human embryonic stem cells CCTL14 line - comparison of low and high number passage
Year: 2009, type: Conference abstract
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Charakterizace delece 22q11 u pacientů s chronickou lymfocytární leukémií.
Year: 2009, type: Conference abstract
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Charakterizace exprese microRNA u CLL pacientů s delecemi 17p13 a 22q11.
Year: 2009, type: Conference abstract